EB06277-T Goat Anti-TCP1 Antibody - Trial Size Price: $100.00 / €100,00 / £75.00 Size: 20µg specific antibody in 40µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info EB06277 Goat Anti-TCP1 Antibody Price: $285.00 / €292,50 / £195.00 Size: 100µg specific antibody in 200µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: TCP1; t-complex 1; CCT-alpha; CCT1; CCTa; D6S230E; TCP-1-alpha; T-complex protein 1; T-complex protein 1, alpha subunit; tailless complex polypeptide 1 Official Symbol: TCP1 Accession Number(s): NP_110379.2 Human GeneID(s): 6950 Gene Ontology Terms: chaperone; protein folding; cytosol Immunogen Peptide with sequence C-SYEDAVHSGALND, from the C Terminus of the protein sequence according to NP_110379.2. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 60kDa band observed in Hela, Human Lung and Human Testis lysates (predicted MW of 60kDa according to NP_110379.2). Recommended for use at 0.5-2µg/ml. Immunohistochemistry: In paraffin embedded Human Testis shows only cytoplasmic staining in some germ cells, and both cytoplasmic and nuclear staining in other germ cells with unstained nucleoli. Recommended concentration, 1-2µg/ml.Species ReactivityTested: Human Expected from sequence similarity: Human, Chimpanzee, Macaque Background ReferenceYaffe MB, Farr GW, Miklos D, Horwich AL, Sternlicht ML, Sternlicht H. TCP1 complex is a molecular chaperone in tubulin biogenesis. Nature. 1992 Jul 16;358(6383):245-8. PMID: 1630491
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For Research purposes only. Not for diagnostic or in vivo use. © Copyright Everest Biotech Ltd 2009 | Elite Grade Product 


EB06277 staining (1µg/ml) of Human Lung lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

EB06277 (10µg/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. |