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Functional Clustering

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  • Secondary Antibodies

  • Rabbit anti-Goat HRP Conjugated


  • Peptides

  • Immunising Peptides available for all antibodies
  • Everest Biotech Ltd Goat Anti-TCP1 Antibody
    Online Catalogue > P114-RHO-GEF to ZNF9 > Goat Anti-TCP1 Antibody

    EB06277-T Goat Anti-TCP1 Antibody - Trial Size
    Price: $100.00 / €100,00 / £75.00
    Size: 20µg specific antibody in 40µl
    EU Availability: In stock
    US Availability: In stock
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    EB06277 Goat Anti-TCP1 Antibody
    Price: $285.00 / €292,50 / £195.00
    Size: 100µg specific antibody in 200µl
    EU Availability: In stock
    US Availability: In stock
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    Ordering Information

    As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.

    Target Protein

    Principal Names: TCP1; t-complex 1; CCT-alpha; CCT1; CCTa; D6S230E; TCP-1-alpha; T-complex protein 1; T-complex protein 1, alpha subunit; tailless complex polypeptide 1
    Official Symbol: TCP1
    Accession Number(s): NP_110379.2
    Human GeneID(s): 6950
    Gene Ontology Terms: chaperone; protein folding; cytosol

    Immunogen

    Peptide with sequence C-SYEDAVHSGALND, from the C Terminus of the protein sequence according to NP_110379.2.

    Purification + Storage

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    Aliquot and store at -20°C. Minimize freezing and thawing.

    Applications Tested

    Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 60kDa band observed in Hela, Human Lung and Human Testis lysates (predicted MW of 60kDa according to NP_110379.2). Recommended for use at 0.5-2µg/ml.
    Immunohistochemistry: In paraffin embedded Human Testis shows only cytoplasmic staining in some germ cells, and both cytoplasmic and nuclear staining in other germ cells with unstained nucleoli. Recommended concentration, 1-2µg/ml.

    Species Reactivity

    Tested: Human
    Expected from sequence similarity: Human, Chimpanzee, Macaque

    Background Reference

    Yaffe MB, Farr GW, Miklos D, Horwich AL, Sternlicht ML, Sternlicht H.
    TCP1 complex is a molecular chaperone in tubulin biogenesis.
    Nature. 1992 Jul 16;358(6383):245-8.
    PMID: 1630491
     


    Adobe PDF Version of Datasheet


    For Research purposes only. Not for diagnostic or in vivo use.

    © Copyright Everest Biotech Ltd 2009

    Elite Grade Product

    Elite Grade Antibodies

    Trial Size Available


    EB06277 Goat Anti-TCP1 Antibody

    EB06277 staining (1µg/ml) of Human Lung lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.


    EB06277 Goat Anti-TCP1 Antibody

    EB06277 (10µg/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

    Online Catalogue > P114-RHO-GEF to ZNF9 > Goat Anti-TCP1 Antibody