EB06276 Goat Anti-VPS28 Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100ug specific antibody in 200ul
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: VPS28; vacuolar protein sorting 28 (yeast); vacuolar protein sorting 28 homolog (S. cerevisiae); MGC60323; vacuolar protein sorting 28; yeast class E protein Vps28p homolog
Official Symbol: VPS28
Accession Number(s): NP_057292.1
Human GeneID(s): 51160
Non-Human GeneID(s): 66914 (mouse);
Important Comments: This antibody is expected to recognize isoform 1 (NP_057292.1) only. Immunogen Peptide with sequence C-ESAYNAFNRFLHA, from the C Terminus of the protein sequence according to NP_057292.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:1,000. Western Blot: Approx 24kDa band observed in H460, Human Testis and Brain lysates (predicted MW of 25kDa according to NP_057292.1). Recommended for use at 1-3µg/ml.
Immunohistochemistry: In paraffin embedded Human Testis, stains acrosomal head caps in round spermatids. Recommended concentration, 10µg/ml.Species ReactivityTested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog
Background ReferenceBishop N, Woodman P.
TSG101/mammalian VPS23 and mammalian VPS28 interact directly and are recruited to VPS4-induced endosomes.
J Biol Chem. 2001 Apr 13;276(15):11735-42.
PMID: 11134028
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB06276 staining (1µg/ml) of Human Testis lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB06276 (10µg/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. |
