EB06271 Goat Anti-SH3BP1 Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100ug specific antibody in 200ul
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: SH3BP1; SH3-domain binding protein 1; SH3 domain binding protein 1; OTTHUMP00000028981
Official Symbol: SH3BP1
Accession Number(s): NP_061830.3
Human GeneID(s): 23616
Immunogen Peptide with sequence C-PQPRPRSLASETN, from the C Terminus of the protein sequence according to NP_061830.3. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 70kDa band observed in Human Lung and Kidney lysates (predicted MW of 71kDa according to NP_061830). Recommended for use at 1-3µg/ml.
Immunohistochemistry: In paraffin embedded Human Kidney, stains the cytoplasm of lymphoid cells in the interstitial parenchyma and of lymphocytes in a glomerulus. Recommended concentration, 0.3µg/ml.Species ReactivityTested: Human
Expected from sequence similarity: Human
Background ReferenceDunham I, Shimizu N, Roe BA, Chissoe S, Hunt AR, Collins JE, Bruskiewich R, Beare DM, Clamp M, Smink LJ, Ainscough R, Almeida JP, Babbage A, Bagguley C, Bailey J, Barlow K, Bates KN, Beasley O, Bird CP, Blakey S, Bridgeman AM, Buck D, Burgess J, Burrill WD, O'Brien KP, et al.
The DNA sequence of human chromosome 22.
Nature. 1999 Dec 2;402(6761):489-95.
PMID: 10591208
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB06271 staining (1µg/ml) of Human Brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB06271 (0.3µg/ml) staining of paraffin embedded Human Kidnay. Cytoplasmic staining of lymphocytes A) in the interstitial parenchyma of the kidney. B) in a glomerulus of the kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining. |
