EB06266 Goat Anti-HADHB Antibody
Price: $185.00 / €187,50 / £125.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: HADHB; HADH; hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit; 3-ketoacyl-Coenzyme A thiolase; hydroxyacyl-Coenzyme A dehydrogenase; MITOCHONDRIAL TRIFUNCTIONAL PROTEIN, BETA SUBUNIT
Official Symbol: HADHB
Accession Number(s): NP_000174
Human GeneID(s): 3032
Gene Ontology Terms: acetyl-CoA C-acyltransferase; enoyl-CoA hydratase; 3-hydroxyacyl-CoA dehydrogenase; fatty acid beta-oxidation; mitochondrial membrane; mitochondrion
Immunogen Peptide with sequence TILTYPFKNLPT-C, from the N Terminus of the protein sequence according to NP_000174. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Preliminary experiments gave no signal but low background in Human Lung and Jurkat extracts at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Species ReactivityTested:
Expected from sequence similarity: Human
Background ReferenceAoyama T, Wakui K, Orii KE, Hashimoto T, Fukushima Y.
Fluorescence in situ hybridization mapping of the alpha and beta subunits (HADHA and HADHB) of human mitochondrial fatty acid beta-oxidation multienzyme complex to 2p23 and their evolution.
Cytogenet Cell Genet. 1997;79(3-4):221-4.
PMID: 9605857
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Aspiring Grade Product 
Please note, this product is one of a range of Aspiring Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot (see Application Notes below). However no additional claims are made for their ability to recognise native protein in any application. |
