EB06233 Goat Anti-KPNA2 / IPOA1 Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100ug specific antibody in 200ul
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US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: KPNA2; IPOA1 QIP2; RCH1; SRP1alpha; karyopherin alpha 2 (RAG cohort 1, importin alpha 1); RAG cohort 1; importin alpha 1
Official Symbol: KPNA2
Accession Number(s): NP_002257.1
Human GeneID(s): 3838
Non-Human GeneID(s): 16647 (mouse); 85245 (rat);
Gene Ontology Terms: nuclear localization sequence binding; protein binding; regulation of DNA recombination; G2 phase of mitotic cell cycle; spindle pole body and microtubule cycle (sensu Saccharomyces); NLS-bearing substrate-nucleus import; DNA metabolism; importin, alpha-subunit; nucleoplasm; cytoplasm; nucleus
Immunogen Peptide with sequence C-QVQDGAPGTFNF, from the C Terminus of the protein sequence according to NP_002257.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution >1/32,000. Western Blot: Approx 55-60kDa band observed in Hela lysates (predicted size of approx. 58kDa according to NP_002257.1). Recommended for use at 0.1-0.5µg/ml
Immunofluoresence: in 4% formaldahyde-fixed HeLa staining is mostly cytoplasmic, with some nuclear staining and clear nuclear pore staining.Species ReactivityTested: Human
Expected from sequence similarity: Human, Mouse, Rat
Background ReferenceCuomo CA, Kirch SA, Gyuris J, Brent R, Oettinger MA.
Rch1, a protein that specifically interacts with the RAG-1 recombination-activating protein.
Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):6156-60.
PMID: 8016130
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB06233 staining (0.1µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB06233 staining (top panel and green) of 4% formaldahyde-fixed HeLa with endogenously expressed HistonH2B-GFP fusion as DNA marker (mid panel and red). Data kindly provided by Francisco Iborra, MRC Mol. Haematol. Unit, Oxford. |
