EB06228 Goat Anti-PAR6alpha / PARD6A Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100ug specific antibody in 200ul
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: PARD6A; PAR-6; TAX40; PAR-6A; TIP-40; PAR6alpha; par-6 partitioning defective 6 homolog alpha (C.elegans); Tax interaction protein 40; par-6 (partitioning defective 6, C.elegans) homolog alpha
Official Symbol: PARD6A
Accession Number(s): NP_058644
Human GeneID(s): 50855
Gene Ontology Terms: transcription factor binding; viral life cycle; oncogenesis
Immunogen Peptide with sequence C-GSRIRGDGSGFSL, from the C Terminus of the protein sequence according to NP_058644. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Preliminary experiments gave no signal but low background in Human Brain and Pancreas extracts at up to 1µg/ml.
Immunohistochemistry: In paraffin embedded Human Pancreas, shows strong cytoplasmic staining in rare cells of intralobular ducts. Recommended concentration, 10µg/ml.
Immunohistochemistry: In paraffin embedded Human Heart shows speckled staining on myocardial fibres in transverse section. Recommended concentration, 5-10µg/ml.Species ReactivityTested: Human
Expected from sequence similarity: Human
Background ReferenceJohansson A, Driessens M, Aspenstrom P.
The mammalian homologue of the Caenorhabditis elegans polarity protein PAR-6 is a binding partner for the Rho GTPases Cdc42 and Rac1.
J Cell Sci. 2000 Sep;113 ( Pt 18):3267-75.
PMID: 10954424
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB06228 (10µg/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
EB06228 (5µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
