EB06115 Goat Anti-SPHAR Antibody
Price: $185.00 / €187,50 / £125.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: SPHAR; S-phase response (cyclin-related)
Official Symbol: SPHAR
Accession Number(s): NP_006533
Human GeneID(s): 10638
Immunogen Peptide with sequence C-NVSFMIDLQAAHF, from the C Terminus of the protein sequence according to NP_006533. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:2,000. Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as small as the predicted size of approx. 7.5kDa according to NP_006533. Therefore we cannot recommend an optimal concentration and the product is investigative grade. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Species ReactivityTested:
Expected from sequence similarity: Human
Background ReferenceDigweed M, Gunthert U, Schneider R, Seyschab H, Friedl R, Sperling K.
Irreversible repression of DNA synthesis in Fanconi anemia cells is alleviated by the product of a novel cyclin-related gene.
Mol Cell Biol. 1995 Jan;15(1):305-14.
PMID: 7799938
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Aspiring Grade Product 
Please note, this product is one of a range of Aspiring Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot (see Application Notes below). However no additional claims are made for their ability to recognise native protein in any application. |
