EB06107 Goat Anti-ARHGEF4 Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100ug specific antibody in 200ul
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: ARHGEF4; Rho guanine nucleotide exchange factor (GEF) 4; ASEF; GEF4; STM6; KIAA1112; DKFZp434G2016; APC-stimulated guanine nucleotide exchange factor
Official Symbol: ARHGEF4
Accession Number(s): NP_056135.2; NP_127462.1
Human GeneID(s): 50649
Important Comments: This antibody is expected to recognise both reported isoforms (NP_056135.2; NP_127462.1). Immunogen Peptide with sequence PWEEPAGEKPSCS, from the N Terminus of the protein sequence according to NP_056135.2; NP_127462.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 80kDa band observed in Human Brain lysates and in transfected HEK293 transiently expressing ARHGEF4 (calculated MW of 79.1kDa according to NP_056135.2 and 76.4kDa according to 127462.1). Recommended concentration: 2-4µg/ml.
Species ReactivityTested: Human
Expected from sequence similarity: Human
Background ReferenceThiesen S, Kubart S, Ropers HH, Nothwang HG.
Isolation of two novel human RhoGEFs, ARHGEF3 and ARHGEF4, in 3p13-21 and 2q22.
Biochem Biophys Res Commun. 2000 Jun 24;273(1):364-9.
PMID: 10873612
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB06107 (2µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing ARHGEF4 (RC215591) and probed with EB06107 (mock transfection in first lane), tested by Origene. |
