EB06104-T Goat Anti-ANILLIN / Scraps (C Terminus) Antibody - Trial Size
Price: $100.00 / €100,00 / £75.00
Size: 20µg specific antibody in 40µl
EU Availability: In stock
US Availability: In stock
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EB06104 Goat Anti-ANILLIN / Scraps (C Terminus) Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
Click to add to EURO Shopping Cart (EU customers only)
Click to add to US Dollar Shopping Cart (US customers only)
Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: Scraps; ANILLIN; ANLN; anillin, actin binding protein (scraps homolog, Drosophila); anillin (Drosophila Scraps homolog), actin binding protein
Official Symbol: ANLN
Accession Number(s): NP_061155.2
Human GeneID(s): 54443
Non-Human GeneID(s): 68743 (mouse);
Gene Ontology Terms: actin binding; regulation of exit from mitosis; septin assembly and septum formation
Immunogen Peptide with sequence WQPDACYKPIGKP, from the C Terminus of the protein sequence according to NP_061155.2. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave an approx 75kDa band in Mouse Brain lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 124kDa according to NP_061155.2. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Immunohistochemistry: In paraffin embedded Human Kidney shows staining of nuclei in some cells of renal tubules.. Recommended concentration, 3-10µg/ml.Species ReactivityTested: Human
Expected from sequence similarity: Human, Mouse
Background ReferenceOegema K, Savoian MS, Mitchison TJ, Field CM.
Functional analysis of a human homologue of the Drosophila actin binding protein anillin suggests a role in cytokinesis.
J Cell Biol. 2000 Aug 7;150(3):539-52.
PMID: 10931866
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For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB06104 (10µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. |
