EB06098 Goat Anti-SAE2 / UBA2 Antibody
Price: $185.00 / €187,50 / £125.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: UBA2; SAE2; SUMO-1 activating enzyme subunit 2
Official Symbol: SAE2
Accession Number(s): NP_005490
Human GeneID(s): 10054
Non-Human GeneID(s): 50995 (mouse);
Gene Ontology Terms: ubiquitin-like activating enzyme activity; ubiquitin activating enzyme activity; enzyme activator activity; catalytic activity; ubiquitin cycle
Immunogen Peptide with sequence ALSRGLPRELAEA-C, from the N Terminus of the protein sequence according to NP_005490. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments showed an approx. 35kDa band in Human Testis and Brain extracts at 1ug of primary, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 74KDa according to NP_005490. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any splice variants/modified forms been reported?
Species ReactivityTested:
Expected from sequence similarity: Human, Mouse
Background ReferenceDesterro JM, Rodriguez MS, Kemp GD, Hay RT.
Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1.
J Biol Chem. 1999 Apr 9;274(15):10618-24.
PMID: 10187858
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Aspiring Grade Product 
Please note, this product is one of a range of Aspiring Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot (see Application Notes below). However no additional claims are made for their ability to recognise native protein in any application. |
