EB06097 Goat Anti-SAE1 / AOS1 Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: SAE1; AOS1; SUA1; HSPC140; SUMO-1 activating enzyme subunit 1; sentrin/SUMO-activating protein AOS1; SUMO-1 activating enzyme E1 N subunit; ubiquitin-like protein SUMO-1 activating enzyme
Official Symbol: SAE1
Accession Number(s): NP_005491
Human GeneID(s): 10055
Gene Ontology Terms: ubiquitin-like conjugating enzyme activity; enzyme activator activity; catalytic activity
Immunogen Peptide with sequence MKGNGIVECLGPK, from the C Terminus of the protein sequence according to NP_005491. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 40kDa band observed in Jurkat and 293 cell lysates (predicted MW of 40kDa according to NP_005491). Recommended for use at 1µg/ml.
Species ReactivityTested: Human
Expected from sequence similarity: Human
Background ReferenceDesterro JM, Rodriguez MS, Kemp GD, Hay RT.
Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1.
J Biol Chem. 1999 Apr 9;274(15):10618-24.
PMID: 10187858
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB06097 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
