EB06016 Goat Anti-FOXH1 / FAST1 Antibody Price: $185.00 / €187,50 / £125.00 Size: 100µg specific antibody in 200µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: FOXH1; forkhead box H1; FAST1; FAST-1; Human homolog of Xenopus forkhead activin signal transducer-1 Official Symbol: FOXH1 Accession Number(s): NP_003914 Human GeneID(s): 8928 Gene Ontology Terms: transcription co-activator activity; transcription factor activity; regulation of transcription, DNA-dependent; signal transduction; nucleus Immunogen Peptide with sequence GPCSGSRLGPPEAE, from the N Terminus of the protein sequence according to NP_003914. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave no signal but low background in Human Skeletal Muscle and A431 lysates at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Species ReactivityTested: Expected from sequence similarity: Human Background ReferenceZhou S, Zawel L, Lengauer C, Kinzler KW, Vogelstein B. Characterization of human FAST-1, a TGF beta and activin signal transducer. Mol Cell. 1998 Jul;2(1):121-7. PMID: 9702198
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For Research purposes only. Not for diagnostic or in vivo use. © Copyright Everest Biotech Ltd 2009 | Aspiring Grade Product  Please note, this product is one of a range of Aspiring Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot (see Application Notes below). However no additional claims are made for their ability to recognise native protein in any application. |