EB05935 Goat Anti-Amisyn / STXBP6 Antibody Price: $185.00 / €187,50 / £125.00 Size: 100µg specific antibody in 200µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: STXBP6; syntaxin binding protein 6 (amisyn); amisyn; HSPC156; amisyn; syntaxin binding protein 6 Official Symbol: STXBP6 Accession Number(s): NP_054897 Human GeneID(s): 29091 Non-Human GeneID(s): 217517 (mouse); Immunogen Peptide with sequence SAKSAISKEIFAP-C, from the N Terminus of the protein sequence according to NP_054897. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: Preliminary experiments gave bands at both approx 25kDa and approx 45kDa in Human Brain extracts at 0.1µg/ml. Please note that currently we cannot find an explanation in the literature for the 45kDa band we observe given the predicted size of approx. 24Kda according to NP_054897. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any splice variants/modified forms been reported?
Species ReactivityTested: Expected from sequence similarity: Human, Mouse, Rat Background ReferenceScales SJ, Hesser BA, Masuda ES, Scheller RH. Amisyn, a novel syntaxin-binding protein that may regulate SNARE complex assembly. J Biol Chem. 2002 Aug 2;277(31):28271-9. PMID: 12145319
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For Research purposes only. Not for diagnostic or in vivo use. © Copyright Everest Biotech Ltd 2009 | Aspiring Grade Product  Please note, this product is one of a range of Aspiring Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot (see Application Notes below). However no additional claims are made for their ability to recognise native protein in any application. |