EB05932 Goat Anti-TRIM5 / RNF88 Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100ug specific antibody in 200ul
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US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: TRIM5; tripartite motif-containing 5; RNF88; TRIM5alpha; tripartite motif protein 5; tripartite motif protein TRIM5
Official Symbol: TRIM5
Accession Number(s): NP_149023.1; NP_149083.1; NP_149084.1
Human GeneID(s): 85363
Important Comments: This antibody is expected to recognise all three human isoforms of this protein. Immunogen Peptide with sequence ASGILVNVKEEVTC, from the N Terminus of the protein sequence according to NP_149023.1; NP_149083.1; NP_149084.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 37kDa band observed in Human Brain lysates (calculated MW of 37.7kDa according to NP_149084.1, isoform delta). An additional faint 40kDa band consistent with calculated MW of 40.1kDa according to NP_149083.1 (isoform gamma) is also observed. Recommended concentration: 1-3µg/ml.
Immunohistochemistry: In paraffin embedded Human Tonsil, stains a group of cells in the interfollicular area, below the epithelium of tonsillar criptae and weak in germinal centre. Recommended concentration, 1.0µg/ml.Species ReactivityTested: Human
Expected from sequence similarity: Human
Background ReferenceReymond A, Meroni G, Fantozzi A, Merla G, Cairo S, Luzi L, Riganelli D, Zanaria E, Messali S, Cainarca S, Guffanti A, Minucci S, Pelicci PG, Ballabio A.
The tripartite motif family identifies cell compartments.
EMBO J. 2001 May 1;20(9):2140-51.
PMID: 11331580
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB05932 staining (1µg/ml) of Human Brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB05932 (1µg/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining. A) positive cells next to a secondary follicle, B) positive cells below tonsillar epithelium. |
