EB05883 Goat Anti-MURF3 / TRIM54 Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: TRIM54; MURF-3; tripartite motif-containing 54; RNF30; MURF; ring finger protein 30; muscle-specific RING-finger protein 3; muscle-specific RING-finger protein homolog
Official Symbol: TRIM54
Accession Number(s): NP_115935
Human GeneID(s): 57159
Immunogen Peptide with sequence C-GSAGPEEERPDGP, from the C Terminus of the protein sequence according to NP_115935. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: experiments gave bands at approx 45kDa and 38kDa in human skeletal muscle lysate after 1µg/ml antibody staining. These bands correspond to the reported isoforms with calculated MWs of 43.1kDa according to NP_115935, and 38.6kDa according to NP_912730. Recommended concentration: 1-3µg/ml.
Species ReactivityTested: Human
Expected from sequence similarity: Human
Background ReferenceCentner T, Yano J, Kimura E, McElhinny AS, Pelin K, Witt CC, Bang ML, Trombitas K, Granzier H, Gregorio CC, Sorimachi H, Labeit S.
Identification of muscle specific ring finger proteins as potential regulators of the titin kinase domain.
J Mol Biol. 2001 Mar 2;306(4):717-26.
PMID: 11243782
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB05883 staining (1ug/ml) of human muscle lysate (RIPA buffer, 35ug total\nprotein per lane). Primary incubated for 1 hour. Detected by western blot\nusing chemiluminescence. |
