EB05879-T Goat Anti-MURF1 / TRIM63 (N Term) Antibody - Trial Size
Price: $100.00 / €100,00 / £75.00
Size: 20µg specific antibody in 40µl
EU Availability: In stock
US Availability: In stock
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EB05879 Goat Anti-MURF1 / TRIM63 (N Term) Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
Click to add to EURO Shopping Cart (EU customers only)
Click to add to US Dollar Shopping Cart (US customers only)
Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: MURF1; MURF-1; RNF28; ring finger protein 28; IRF; SMRZ; MURF2; FLJ32380; iris ring finger protein; muscle specific ring finger protein 2; striated muscle RING zinc finger protein
Official Symbol: TRIM63
Accession Number(s): NP_115977.2
Human GeneID(s): 84676
Gene Ontology Terms: zinc ion binding; signal transducer activity; signal transduction; kinesin complex; microtubule; cytoplasm; nucleus
Immunogen Peptide with sequence DYKSSLIQDGNPM-C, from the N Terminus of the protein sequence according to NP_115977.2. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx 60kDa band in Human Skeletal Muscle lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.2kDa according to NP_115977.2. The 60kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Immunohistochemistry: In paraffin embedded Human Skeletal Muscle shows strong staining in rare nuclei, and weak staining of muscle fibres in longitudinal section.Species ReactivityTested: Human
Expected from sequence similarity: Human, Dog
Background ReferenceDai KS, Liew CC.
A novel human striated muscle RING zinc finger protein, SMRZ, interacts with SMT3b via its RING domain.
J Biol Chem. 2001 Jun 29;276(26):23992-9.
PMID: 11283016
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For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB05879 (1.25µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
