EB05868 Goat Anti-BERP / RNF22 Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100ug specific antibody in 200ul
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: BERP; RNF22; TRIM3; tripartite motif-containing 3; HAC1; RNF97; ring finger protein 22; brain expressed ring finger; tripartite motif protein TRIM3
Official Symbol: TRIM3
Accession Number(s): NP_006449.2; NP_150594.2
Human GeneID(s): 10612
Non-Human GeneID(s): 55992 (mouse); 83616 (rat);
Gene Ontology Terms: cytoplasm; neurogenesis; protein C-terminus binding
Important Comments: Both variants (NP_006449.2; NP_150594.2;) represent the same protein. This antibody is expected to recognise all three human isoforms of this protein. Immunogen Peptide with sequence AKREDSPGPEVQP-C, from the N Terminus of the protein sequence according to NP_006449.2; NP_150594.2. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 80kDa band observed in Mouse Brain lysates (calculated MW of 80.8kDa according to NP_006449.2 and NP_150594.2). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Species ReactivityTested: Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog
Background ReferenceEl-Husseini AE, Fretier P, Vincent SR.
Cloning and characterization of a gene (RNF22) encoding a novel brain expressed ring finger protein (BERP) that maps to human chromosome 11p15.5.
Genomics. 2001 Feb 1;71(3):363-7.
PMID: 11170753
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB05868 staining (1ug/ml) of Mouse Brain (RIPA buffer, 35ug total\nprotein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
HEK293 overexpressing TRIM3 (RC211928) and probed with EB05868 (mock transfection in first lane), tested by Origene. |
