EB05823 Goat Anti-MID2 / TRIM1 Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100ug specific antibody in 200ul
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US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: MID2; TRIM1; midline 2; FXY2; midin 2; tripartite motif protein 1
Official Symbol: MID2
Accession Number(s): NP_036348.1; NP_438112.1
Human GeneID(s): 11043
Non-Human GeneID(s): 23947 (mouse);
Gene Ontology Terms: microtubule associated protein; embryogenesis and morphogenesis; cell shape and cell size control
Important Comments: This antibody is expected to recognise both human isoforms of this protein. Immunogen Peptide with sequence QESPYVSGMKTCH, from the C Terminus of the protein sequence according to NP_036348.1; NP_438112.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 80kDa band observed in mouse heart lysates (calculated MW of 81.3kDa according to NP_036348.1 and 77.9kDa according to NP_438112.1). Recommended concentration: 1-3µg/ml.
Immunohistochemistry: In paraffin embedded Human Kidney shows strong staining in cytoplasm and brush border of some epithelial cells of proximal convoluted tubules. Recommended concentration, 5-10µg/ml.Species ReactivityTested: Mouse, Human
Expected from sequence similarity: Human, Mouse
Background ReferenceBuchner G, Montini E, Andolfi G, Quaderi N, Cainarca S, Messali S, Bassi MT, Ballabio A, Meroni G, Franco B.
MID2, a homologue of the Opitz syndrome gene MID1: similarities in subcellular localization and differences in expression during development.
Hum Mol Genet. 1999 Aug;8(8):1397-407.
PMID: 10400986
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB05823 (1µg/ml) staining of mouse heart lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB05823 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
