EB05819 Goat Anti-CHFR Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: CHFR; checkpoint with forkhead and ring finger domains; FLJ10796
Official Symbol: CHFR
Accession Number(s): NP_060693
Human GeneID(s): 55743
Non-Human GeneID(s): 231600 (mouse); 288734 (rat);
Immunogen Peptide with sequence KFNHICEQTRFKN, from the C Terminus of the protein sequence according to NP_060693. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave an approx 80kDa band in human brain lysates at 0.5ug/ml, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 72kDda according to NP_060693. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Immunohistochemistry: In paraffin embedded human brain (cerebellum) shows stained Purkinje cells. Recommended concentration, 2.5µg/ml.Species ReactivityTested: Human
Expected from sequence similarity: Human, Mouse, Rat
Background ReferenceScolnick DM, Halazonetis TD.
Chfr defines a mitotic stress checkpoint that delays entry into metaphase.
Nature. 2000 Jul 27;406(6794):430-5.
PMID: 10935642
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB05819 (2.5µg/ml) staining of paraffin embedded human brain (cerebellum). Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
