EB05812 Goat Anti-ARIH1 / HHARI Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: ARIH1; HHARI; ARI; HARI; UBCH7BP; ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila); ariadne homolog; ariadne, Drosophila, homolog of
Official Symbol: ARIH1
Accession Number(s): NP_005735
Human GeneID(s): 25820
Non-Human GeneID(s): 23806 (mouse);
Gene Ontology Terms: ubiquitin-dependent protein catabolism; kinesin complex; ubiquitin ligase complex; enzyme binding; ubiquitin-like-protein ligase activity
Immunogen Peptide with sequence C-HEGYEKDLWEYIED, from the C Terminus of the protein sequence according to NP_005735. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: Pass > 1/32000. Western Blot: Approx 65kDa band seen in U937 lysate [Predicted MW of approx. 61kDa according to NP_005735]. Recommended for use at 0.2-1µg/ml.
Species ReactivityTested: Human
Expected from sequence similarity: Human, Mouse
Background ReferenceMoynihan TP, Ardley HC, Nuber U, Rose SA, Jones PF, Markham AF, Scheffner M, Robinson PA.
The ubiquitin-conjugating enzymes UbcH7 and UbcH8 interact with RING finger/IBR motif-containing domains of HHARI and H7-AP1.
J Biol Chem. 1999 Oct 22;274(43):30963-8.
PMID: 10521492
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB05812 staining (0.5µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
