EB05810-T Goat Anti-KAP1 / TRIM28 Antibody - Trial Size
Price: $100.00 / €100,00 / £75.00
Size: 20µg specific antibody in 40µl
EU Availability: In stock
US Availability: In stock
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EB05810 Goat Anti-KAP1 / TRIM28 Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
Click to add to EURO Shopping Cart (EU customers only)
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Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: TRIM28; KAP1; TF1B; RNF96; TIF1B; tripartite motif-containing 28; KRAB-associated protein 1; nuclear corepressor KAP-1; transcriptional intermediary factor 1-beta
Official Symbol: TRIM28
Accession Number(s): NP_005753
Human GeneID(s): 10155
Gene Ontology Terms: transcription co-repressor activity; zinc ion binding; transcription factor activity; regulation of transcription from Pol II promoter; nucleus
Immunogen Peptide with sequence C-SSQELSGGPGDGP, from the C Terminus of the protein sequence according to NP_005753. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedWestern Blot: Approx 100-120kDa band seen in HepG2 lysate. Recommended for use at 0.2-1µg/ml (Predicted MW approx 92kDa according to NP_005753).
Immunofluorescence: Successfully used in on mouse embryo at the late zygote stage, 5ug/ml (personal communication).Species ReactivityTested: Human, Mouse
Expected from sequence similarity: Human, Mouse
Background ReferenceFriedman JR, Fredericks WJ, Jensen DE, Speicher DW, Huang XP, Neilson EG, Rauscher FJ 3rd.
KAP-1, a novel corepressor for the highly conserved KRAB repression domain.
Genes Dev. 1996 Aug 15;10(16):2067-78.
PMID: 8769649
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB05810 staining (0.5µg/ml) of HepG2 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
