EB05735-T Goat Anti-PDIA3 / ERp57 / GRP58 Antibody - Trial Size Price: $100.00 / €100,00 / £75.00 Size: 20µg specific antibody in 40µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info EB05735 Goat Anti-PDIA3 / ERp57 / GRP58 Antibody Price: $285.00 / €292,50 / £195.00 Size: 100µg specific antibody in 200µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: protein disulfide isomerase family A, member 3; ERp57; ERp60; ERp61; GRP57; GRP58; HsT17083; P58; PI-PLC; 58 kDa microsomal protein; endoplasmic reticulum P58; glucose regulated protein, 58kDa; phospholipase C-alpha; protein disulfide isomerase-associated 3 Official Symbol: PDIA3 Accession Number(s): NP_005304.3 Human GeneID(s): 2923 Non-Human GeneID(s): 14827 (mouse); 29468 (rat); Gene Ontology Terms: ER retention; phospholipase C; signal transduction; protein-nucleus import; cysteine-type endopeptidase; protein disulfide isomerase Immunogen Peptide with sequence C-EEKPKKKKKAQEDL, from the C Terminus of the protein sequence according to NP_005304.3. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 55kDa band observed in human hepatoblastoma HepG2 lysates (calculated MW of 56.8kDa according to NP_005304.3). Recommended concentration: 1-3µg/ml.
Species ReactivityTested: Human Expected from sequence similarity: Human, Mouse, Rat, Rabbit, Hamster Background ReferenceKoivunen P, Horelli-Kuitunen N, Helaakoski T, Karvonen P, Jaakkola M, Palotie A, Kivirikko KI. Structures of the human gene for the protein disulfide isomerase-related polypeptide ERp60 and a processed gene and assignment of these genes to 15q15 and 1q21. Genomics. 1997 Jun 15;42(3):397-404. PMID: 9205111
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EB05735 staining (1µg/ml) of HepG2 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |