EB05677-T Goat Anti-H11 / HSP22 Antibody - Trial Size Price: $100.00 / €100,00 / £75.00 Size: 20µg specific antibody in 40µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info EB05677 Goat Anti-H11 / HSP22 Antibody Price: $285.00 / €292,50 / £195.00 Size: 100µg specific antibody in 200µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: HSPB8; heat shock 22kDa protein 8; CMT2L; DHMN2; E2IG1; H11; HMN2; HMN2A; HSP22; Charcot-Marie-Tooth disease, spinal; heat shock 27kDa protein 8; hereditary motor neuropathy, distal; protein kinase H11; small stress protein-like protein HSP22; spinal muscular atrophy, distal, adult, autosomal dominant Official Symbol: HSPB8 Accession Number(s): NP_055180.1 Human GeneID(s): 26353 Gene Ontology Terms: protein serine/threonine kinase activity; heat shock protein activity; biological_process unknown; cellular_component unknown; transferase activity Immunogen Peptide with sequence NELPQDSQEVTCT, from the C Terminus of the protein sequence according to NP_055180.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: Approx 20-25kDa band seen in human skeletal muscle lysate [Predicted MW of approx. 22kDa according to NP_055180]. Recommended for use at 0.5-1µg/ml.
Species ReactivityTested: Human Expected from sequence similarity: Human Background ReferenceBenndorf R, Sun X, Gilmont RR, Biederman KJ, Molloy MP, Goodmurphy CW, Cheng H, Andrews PC, Welsh MJ. HSP22, a new member of the small heat shock protein superfamily, interacts with mimic of phosphorylated HSP27 ((3D)HSP27). J Biol Chem. 2001 Jul 20;276(29):26753-61. PMID: 11342557
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EB05677 staining (1µg/ml) of human muscle lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |