EB05676 Goat Anti-BOB1 / OCAB / OBF1 Antibody Price: $285.00 / €292,50 / £195.00 Size: 100µg specific antibody in 200µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: BOB1; OBF1; POU2AF1; OBF-1; POU domain, class 2, associating factor 1 Official Symbol: POU2AF1 Accession Number(s): NP_006226 Human GeneID(s): 5450 Non-Human GeneID(s): 18985 (mouse); Gene Ontology Terms: transcription co-activator activity; cell growth and/or maintenance; humoral immune response; regulation of transcription, DNA-dependent; transcription from Pol II promoter; nucleus Immunogen Peptide with sequence C-DAYALNHTLSVEGF, from the C Terminus of the protein sequence according to NP_006226. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedWestern blot: Approx 30kDa band seen in mouse spleen lysate (Predicted MW of 28kDa according to NP_006226). Recommended for use at 1-3µg/ml.
Species ReactivityTested: Mouse Expected from sequence similarity: Human, Mouse, Rat Background ReferenceStrubin M, Newell JW, Matthias P. OBF-1, a novel B cell-specific coactivator that stimulates immunoglobulin promoter activity through association with octamer-binding proteins. Cell. 1995 Feb 10;80(3):497-506. PMID: 7859290
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For Research purposes only. Not for diagnostic or in vivo use. © Copyright Everest Biotech Ltd 2009 | Elite Grade Product 

EB05676 staining (2µg/ml) of mouse spleen lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |