EB05674 Goat Anti-ACADM Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100ug specific antibody in 200ul
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: ACADM; acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain; RP4-682C21.1; ACAD1; MCAD; MCADH; medium-chain acyl-CoA dehydrogenase
Official Symbol: ACADM
Accession Number(s): NP_000007.1
Human GeneID(s): 34
Gene Ontology Terms: mitochondrion; energy pathways; fatty acid beta-oxidation
Immunogen Peptide with sequence C-RLIVAREHIDKYKN, from the C Terminus of the protein sequence according to NP_000007.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 45kDa band observed in Human Kidney and Heart lysates (calculated MW of 46.6kDa according to NP_000007.1). In transfected HEK293 transiently expressing ACADM bands of approx. 48kDa and 50kDa are observed. These bands are not observed in the non-transfected HEK293. Recommended for use at 0.01-0.1µg/ml.
Species ReactivityTested: Human
Expected from sequence similarity: Human
Product Specific ReferenceThe goat polyclonal antibody used in this paper was manufactured by us:
Powelka et al, Suppression of oxidative metabolism and mitochondrial biogenesis by the transcriptional corepressor RIP140 in mouse adipocytes. J Clin Invest. 2006 Jan;116(1):125-36. Epub 2005 Dec 22.
PMID: 16374519
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB05674 staining (0.05µg/ml) of Human Heart lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
HEK293 overexpressing ACADM (RC202798) with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with EB05674 in the right panel (mock transfection in first and last lanes), tested by Origene. |
