EB05668 Goat Anti-BRF2 Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: BRF2; BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like; BRFU; FLJ11052; TFIIIB50; transcription factor IIB- related factor, TFIIIB50; RNA polymerase III transcription initiation factor BRFU
Official Symbol: BRF2
Accession Number(s): NP_060780
Human GeneID(s): 55290
Gene Ontology Terms: translation initiation factor activity;
Immunogen Peptide with sequence C-QAARQAATSVPNPP, from the C Terminus of the protein sequence according to NP_060780. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 50kDa band observed in Hela and Human Placenta lysates (predicted MW of 48kDa according to NP_060780). Recommended for use at 1-3µg/ml.
Species ReactivityTested: Human
Expected from sequence similarity: Human
Background ReferenceSchramm L, Pendergrast PS, Sun Y, Hernandez N.
Different human TFIIIB activities direct RNA polymerase III transcription from TATA-containing and TATA-less promoters.
Genes Dev. 2000 Oct 15;14(20):2650-63.
PMID: 11040218
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB05668 staining (1.5µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
