EB05639 Goat Anti-Dynactin Antibody
Price: $300.00 / €300,00 / £205.00
Size: 100ug specific antibody in 200ul
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: DCTN1; dynactin 1 (p150, glued homolog, Drosophila); P135; DP-150; DAP-150; p150-glued; p150, Glued (Drosophila) homolog; 150 kDa dynein-associated polypeptide; dynactin 1 (p150, Glued (Drosophila) homolog); HMN7B; OTTHUMP00000202491; dynactin 1; p150, Glued homolog
Official Symbol: DCTN1
Accession Number(s): NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1
Human GeneID(s): 1639
Non-Human GeneID(s): 13191 (mouse);
Gene Ontology Terms: mitosis; cytoplasm; neurogenesis
Important Comments: This antibody is expected to recognise all reported isoforms (NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1). Immunogen Peptide with sequence C-QEQLHQLHSRLIS, from the C Terminus of the protein sequence according to NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:128000.
Western Blot: Approx 150kDa band observed in cell lline A549 and Human Testis lysates (calculated MW of 142kDa according to NP_004073.2). Recommended for use at 0.5-2µg/ml.
Immunohistochemistry: In paraffin embedded Human Cerebellum shows pixulate staining of cytoplasm in neuronal cells. In paraffin embedded Human Testis shows cytoplasm staining in cells of the seminiferous tubules. Recommended concentration, 2-4µg/ml.
Species ReactivityTested: Human
Expected from sequence similarity: Human, Mouse
Background ReferenceHolzbaur EL, Tokito MK.
Localization of the DCTN1 gene encoding p150Glued to human chromosome 2p13 by fluorescence in situ hybridization.
Genomics. 1996 Feb 1;31(3):398-9. No abstract available.
PMID: 8838327
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB05639 staining (1µg/ml) of Human Testis lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB05639 (2.5µg/ml) staining of A) paraffin embedded Human Cerebellum and B) paraffin embedded Human Testis. A) & B) Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
