EB05543 Goat Anti-SAP130 / SF3B3 Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: SF3B3; splicing factor 3b, subunit 3, 130kDa; RSE1; SAP130; SF3B130; KIAA0017; STAF130; pre-mRNA splicing factor SF3b, 130 kDa subunit; spliceosome-associated protein 130; splicing factor 3b, subunit 3
Official Symbol: SF3B3
Accession Number(s): NP_036558.3
Human GeneID(s): 23450
Non-Human GeneID(s): 101943 (mouse);
Gene Ontology Terms: pre-mRNA splicing factor activity; small nuclear ribonucleoprotein; mRNA processing; mRNA splicing; protein complex assembly; spliceosome complex
Immunogen Peptide with sequence C-VSKKLEDIRTRYAF, from the C Terminus of the protein sequence according to NP_036558.3. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 150kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 136kDa according to NP_036558.3). Recommended concentration: 0.1-0.3µg/ml.
Species ReactivityTested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog
Background ReferenceDas BK, Xia L, Palandjian L, Gozani O, Chyung Y, Reed R.
Characterization of a protein complex containing spliceosomal proteins SAPs 49, 130, 145, and 155.
Mol Cell Biol 1999 Oct;19(10):6796-802
PMID: 10490618
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB05543 staining (0.1µg/ml) of nuclear lysate from HeLa cells (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
