EB05540 Goat Anti-SF3B4 / SAP49 Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
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US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: SF3B4; splicing factor 3b, subunit 4, 49kD; SAP49; SF3B49; MGC10828; spliceosome-associated protein (U2 snRNP); spliceosomal protein; splicing factor 3b, subunit 4
Official Symbol: SF3B4
Accession Number(s): NP_005841.1
Human GeneID(s): 10262
Non-Human GeneID(s): 107701 (mouse);
Gene Ontology Terms: nucleus; spliceosome; mRNA splicing; mRNA processing; pre-mRNA splicing factor
Immunogen Peptide with sequence AAGPISERNQDAT-C, from the N Terminus of the protein sequence according to NP_005841.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 48kDa band observed in Human HeLa lysates (calculated MW of 44.4kDa according to NP005841.1). Recommended concentration: 0.1-0.3µg/ml.
Species ReactivityTested: Human
Expected from sequence similarity: Human, Mouse, Rat
Background ReferenceChampion-Arnaud P, Reed R.
The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch site.
Genes Dev 1994 Aug 15;8(16):1974-83
PMID: 7958871
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB05540 staining (0.1µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
