EB05535 Goat Anti-RANBP8 / IPO8 Antibody
Price: $185.00 / €187,50 / £125.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: IPO8; importin 8; RANBP8; RAN-binding protein 8
Official Symbol: IPO8
Accession Number(s): NP_006381.2
Human GeneID(s): 10526
Gene Ontology Terms: RAN protein binding; protein transporter activity; protein-nucleus import, docking; signal transduction; nuclear pore; cytoplasm; nucleus
Immunogen Peptide with sequence C-SAFNFGTVPSNN, from the C Terminus of the protein sequence according to NP_006381.2. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx 23kDa band in lysates of cell lines A431, A549 and HeLa after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 120kDa according to NP_006381.2. The 23kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Species ReactivityTested:
Expected from sequence similarity: Human
Background ReferenceGorlich D, Dabrowski M, Bischoff FR, Kutay U, Bork P, Hartmann E, Prehn S, Izaurralde E.
A novel class of RanGTP binding proteins.
J Cell Biol. 1997 Jul 14;138(1):65-80.
PMID: 9214382
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Aspiring Grade Product 
Please note, this product is one of a range of Aspiring Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot (see Application Notes below). However no additional claims are made for their ability to recognise native protein in any application. |
