EB05511 Goat Anti-ELF5 Antibody
Price: $185.00 / €187,50 / £125.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: ELF5; E74-like factor 5 (ets domain transcription factor); ESE2; ESE-2; E74-like factor 5; epithelium-specific Ets transcription factor 2
Official Symbol: ELF5
Accession Number(s): NP_938195.1; NP_001413.1
Human GeneID(s): 2001
Non-Human GeneID(s): 13711 (mouse);
Gene Ontology Terms: cell proliferation; transcription activating factor; transcription from Pol II promoter
Important Comments: This antibody is expected to recognise both reported Human isoforms represented by NP_938195.1 and NP_001413.1. Immunogen Peptide with sequence C-GKNAHGWQEDKL, from the C Terminus of the protein sequence according to NP_938195.1; NP_001413.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Preliminary experiments in Human lysates of Lung, Kidney, Prostate and Testes gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Species ReactivityTested:
Expected from sequence similarity: Human, Mouse, Rat
Background ReferenceOettgen P, Kas K, Dube A, Gu X, Grall F, Thamrongsak U, Akbarali Y, Finger
E, Boltax J, Endress G, Munger K, Kunsch C, Libermann TA.
Characterization of ESE-2, a novel ESE-1-related Ets transcription factor that is restricted to glandular epithelium and differentiated keratinocytes.
J Biol Chem. 1999 Oct 8;274(41):29439-52.
PMID: 10506207
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Aspiring Grade Product 
Please note, this product is one of a range of Aspiring Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot (see Application Notes below). However no additional claims are made for their ability to recognise native protein in any application. |
