EB05441 Goat Anti-SMARCA3 Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: SMARCA3; HLTF; ZBU1; HLTF1; RNF80; HIP116; SNF2L3; HIP116A; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3; SNF2-like 3; sucrose nonfermenting-like 3; helicase-like transcription factor; DNA-binding protein/plasminogen activator inhibitor-1 regulator
Official Symbol: SMARCA3
Accession Number(s): NP_003062.2; NP_620636.1
Human GeneID(s): 6596
Non-Human GeneID(s): 20585 (mouse);
Gene Ontology Terms: ATP binding; DNA binding; regulation of transcription, DNA-dependent; nucleus; ATP dependent helicase activity; hydrolase activity
Important Comments: NP_003062.2 and NP_620636.2 are varients that represent the same protein. Immunogen Peptide with sequence C-KQAKINEIRTLIDL, from the C Terminus of the protein sequence according to NP_003062.2; NP_620636.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedWestern blot: Approx 120kDa and 50kDa bands seen in Jurkat cell lysate. Recommended for use at 0.2-1µg/ml. Please note that the weaker smaller band may represent a novel isoform of this protein and is in agreement with previous Western blot observations (J Biol Chem 1995 Mar 3;270(9):4575-87).
Species ReactivityTested: Human
Expected from sequence similarity: Human, Mouse
Background ReferenceSheridan PL, Schorpp M, Voz ML, Jones KA.
Cloning of an SNF2/SWI2-related protein that binds specifically to the SPH motifs of the SV40 enhancer and to the HIV-1 promoter.
J Biol Chem. 1995 Mar 3;270(9):4575-87.
PMID: 7876228
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB05441 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
