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  • Everest Biotech Ltd Goat Anti-ARP2/3 subunit 1B Antibody
    Online Catalogue > 14-3-3 sigma to FZD9 > Goat Anti-ARP2/3 subunit 1B Antibody

    EB05435 Goat Anti-ARP2/3 subunit 1B Antibody
    Price: $285.00 / €292,50 / £195.00
    Size: 100µg specific antibody in 200µl
    EU Availability: In stock
    US Availability: In stock
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    Ordering Information

    As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.

    Target Protein

    Principal Names: ARPC1B; actin related protein 2/3 complex, subunit 1B, 41kDa; ARC41; p40-ARC; p41-ARC; ARP2/3 protein complex subunit p41; actin related protein 2/3 complex, subunit 1A (41 kD)
    Official Symbol: ARPC1B
    Accession Number(s): NP_005711
    Human GeneID(s): 10095
    Gene Ontology Terms: cell motility; actin cytoskeleton; Arp2/3 protein complex; cytoskeletal structural protein

    Immunogen

    Peptide with sequence AYHSFLVEPISCH, from the N Terminus of the protein sequence according to NP_005711.

    Purification + Storage

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    Aliquot and store at -20°C. Minimize freezing and thawing.

    Applications Tested

    Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 37kDa band observed in Human Liver lysates (calculated MW of 40.9kDa according to NP_005711). Recommended concentration: 2-4µg/ml. A minor band of unknown identity was also consistently observed at 28kDa.

    Species Reactivity

    Tested: Human
    Expected from sequence similarity: Human, Mouse, Rat

    Background Reference

    Welch MD, DePace AH, Verma S, Iwamatsu A, Mitchison TJ.
    The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly.
    J Cell Biol. 1997 Jul 28;138(2):375-84.
    PMID: 9230079
     


    Adobe PDF Version of Datasheet


    For Research purposes only. Not for diagnostic or in vivo use.

    © Copyright Everest Biotech Ltd 2009

    Elite Grade Product

    Elite Grade Antibodies

    EB05435 Goat Anti-ARP2/3 subunit 1B Antibody

    EB05435 staining (2µg/ml) of Human Liver lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

    Online Catalogue > 14-3-3 sigma to FZD9 > Goat Anti-ARP2/3 subunit 1B Antibody