EB05419 Goat Anti-AIF1 / IBA1 (isoform 1 and 3) Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: AIF1; IBA1; allograft inflammatory factor 1; AIF-1; IRT-1; interferon gamma responsive transcript; ionized calcium-binding adapter molecule; Daintain
Official Symbol: AIF1
Accession Number(s): NP_116573.1; NP_001614.3
Human GeneID(s): 199
Non-Human GeneID(s): 11629 (mouse); 29427 (rat);
Gene Ontology Terms: cell cycle arrest; negative regulation of cell proliferation; response to stress; inflammatory response; nucleus
Important Comments: This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3). Immunogen Peptide with sequence C-TGPPAKKAISELP, from the C Terminus of the protein sequence according to NP_116573.1; NP_001614.3. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx. 17kDa band observed in Human Brain (Frontal Cortex) and Rat Brain lysates (calculated MW of 16.7kDa according to Human NP_001614.3 and of 16.8kDa according to Rat NP_058892.1). Recommended concentration: 0.3-0.5µg/ml.
Species ReactivityTested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat
Background ReferenceAutieri MV, Agrawal N.
IRT-1, a novel interferon-gamma-responsive transcript encoding a growth-suppressing basic leucine zipper protein.
J Biol Chem. 1998 Jun 12;273(24):14731-7.
PMID: 9614071
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB05419 (0.3µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
