EB05417 Goat Anti-GRB7 (N Terminus) Antibody Price: $285.00 / €292,50 / £195.00 Size: 100µg specific antibody in 200µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: GRB7; growth factor receptor-bound protein 7 Official Symbol: GRB7 Accession Number(s): NP_005301 Human GeneID(s): 2886 Non-Human GeneID(s): 14786 (mouse); Gene Ontology Terms: SH3/SH2 adaptor protein activity; EGF receptor signaling pathway; oncogenesis Important Comments: This antibody recognises the longer isoform of this protein in A431 lysate. Immunogen Peptide with sequence ELDLSPPHLSSSPE-C, from the N Terminus of the protein sequence according to NP_005301. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedWestern blot: Approx 60-65kDa band seen in A431 lysate. Recommended for use at 1-3µg/ml.
Species ReactivityTested: Human Expected from sequence similarity: Human Background ReferenceMargolis B, Silvennoinen O, Comoglio F, Roonprapunt C, Skolnik E, Ullrich A, Schlessinger J. High-efficiency expression/cloning of epidermal growth factor-receptor-binding proteins with Src homology 2 domains. Proc Natl Acad Sci U S A. 1992 Oct 1;89(19):8894-8. PMID: 1409582
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For Research purposes only. Not for diagnostic or in vivo use. © Copyright Everest Biotech Ltd 2009 | Elite Grade Product 

EB05417 staining (2µg/ml) of A431 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |