EB05375 Goat Anti-ARHU / WRCH1 Antibody
Price: $185.00 / €187,50 / £125.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: RHOU; ras homolog gene family, member U; ARHU; WRCH1; hG28K; WRCH-1; CDC42L1; FLJ10616; DJ646B12.2; fJ646B12.2; 2310026M05Rik; ras homolog gene family, member U; Ryu GTPase; CDC42-like GTPase; GTP-binding protein like 1; Wnt-1 responsive Cdc42 homolog
Official Symbol: RHOU
Accession Number(s): NP_067028.1
Human GeneID(s): 58480
Gene Ontology Terms: Rho small monomeric GTPase activity; GTP binding; small GTPase mediated signal transduction
Immunogen Peptide with sequence PPQQGDPAFPDRCEA, from the N Terminus of the protein sequence according to NP_067028.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave an approx. 22kDa band in lysates o f cell lines A431, HeLa and HEK293 after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 28.2kDa according to NP_067028.1. The 22kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Species ReactivityTested:
Expected from sequence similarity: Human
Background ReferenceTao W, Pennica D, Xu L, Kalejta RF, Levine AJ.
Wrch-1, a novel member of the Rho gene family that is regulated by Wnt-1.
Genes Dev. 2001 Jul 15;15(14):1796-807.
PMID: 11459829
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Aspiring Grade Product 
Please note, this product is one of a range of Aspiring Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot (see Application Notes below). However no additional claims are made for their ability to recognise native protein in any application. |
