EB05361 Goat Anti-DKK3 / REIC Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: DKK3; REIC; dickkopf homolog 3 (Xenopus laevis); DKK-3; RIG-like 5-6; RIG-like 7-1; dickkopf 3; Dickkopf gene 3; dickkopf (Xenopus laevis) homolog 3; dickkopf homolog 3
Official Symbol: DKK3
Accession Number(s): NP_037385.2; NP_056965.3; NP_001018067.1
Human GeneID(s): 27122
Gene Ontology Terms: embryogenesis and morphogenesis; extracellular space
Important Comments: NP_037385.2; NP_056965.3 and NP_001018067.1 are varients of the same protein. Immunogen Peptide with sequence C-AAAALLGGEEI, from the C Terminus of the protein sequence according to NP_037385.2; NP_056965.3; NP_001018067.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:16,000. Approx. 70kDa band observed in lysates of cell line HEK293 (calculated MW of 38.4kDa according to NP_037385.2, NP_056965.3 and NP_001018067.1). The observed molecular weight corresponds to earlier findings in literature (Krupnik et al, Gene. 1999 Oct 1;238(2):301-13.; PMID: 10570958 ). Recommended concentration: 0.03-0.1µg/ml.
Species ReactivityTested: Human
Expected from sequence similarity: Human
Background ReferenceKrupnik VE, Sharp JD, Jiang C, Robison K, Chickering TW, Amaravadi L, Brown DE, Guyot D, Mays G, Leiby K, Chang B, Duong T, Goodearl AD, Gearing DP, Sokol SY, McCarthy SA.
Functional and structural diversity of the human Dickkopf gene family.
Gene. 1999 Oct 1;238(2):301-13.
PMID: 10570958
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB05361 staining (2µg/ml) of HEK293 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence. |
