EB05356 Goat Anti-SCAP2 / PRAP Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: SCAP2; src family associated phosphoprotein 2; MGC10411; MGC33304; PRAP; RA70; SAPS; SCAP2; SKAP-HOM; SKAP55R; Fyn-associated phosphoprotein SKAP55 homologue; Pyk2/RAFTK-associated protein; src family associated phosphoprotein 2; src kinase-associated phosphoprotein of 55-related protein; src-associated adaptor protein
Official Symbol: SKAP2
Accession Number(s): NP_003921.2
Human GeneID(s): 8935
Gene Ontology Terms: signal transduction; SH3/SH2 adaptor protein; protein complex assembly
Immunogen Peptide with sequence C-GLVPKAYIMEMYDI, from the C Terminus of the protein sequence according to NP_003921.2. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 38kDa band observed in lysates of cell line Jurkat (calculated MW of 41.2kDa according to NP_003921.2). Recommended concentration: 0.5-2µg/ml.
An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Species ReactivityTested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Background ReferenceTakahashi T, Yamashita H, Nagano Y, Nakamura T, Ohmori H, Avraham H, Avraham
S, Yasuda M, Matsumoto M.
Identification and characterization of a novel Pyk2/related adhesion focal
tyrosine kinase-associated protein that inhibits alpha-synuclein
phosphorylation.
J Biol Chem. 2003 Oct 24;278(43):42225-33. Epub 2003 Jul 31
PMID: 12893833
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB05356 (0.5µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
