EB05345-T Goat Anti-APE1 / APEX1 Antibody - Trial Size
Price: $100.00 / €100,00 / £75.00
Size: 20µg specific antibody in 40µl
EU Availability: In stock
US Availability: In stock
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EB05345 Goat Anti-APE1 / APEX1 Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
Click to add to EURO Shopping Cart (EU customers only)
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Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: APE1; APEX1; APEX; APEX nuclease (multifunctional DNA repair enzyme); APE; APX; APEN; HAP1; REF1; REF-1; AP lyase; AP endonuclease class I; apurinic/apyrimidinic exonuclease; multifunctional DNA repair enzyme; DNA-(apurinic or apyrimidinic site) lyase; apurinic/apyrimidinic (abasic) endonuclease; deoxyribonuclease (apurinic or apyrimidinic)
Official Symbol: APEX1
Accession Number(s): NP_001632.2; NP_542379.1; NP_542380.1
Human GeneID(s): 328
Gene Ontology Terms: DNA-(apurinic or apyrimidinic site) lyase activity; uracil DNA N-glycosylase activity; endodeoxyribonuclease activity; transcription co-repressor activity; 3'-5' exonuclease activity; endonuclease activity; DNA binding; transcription co-activator activity; base-excision repair; DNA repair; transcription from Pol II promoter; obsolete; nucleus
Important Comments: NP_001632.2; NP_542379.1 and NP_542380.1 are varients that represent the same protein. Immunogen Peptide with sequence PKRGKKGAVAEDGD-C, from the N Terminus of the protein sequence according to NP_001632.2; NP_542379.1; NP_542380.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 35kDa band observed in Human epidermoid fibroblast A431 and Human cervix epitheloid fibroblast HeLa lysates (calculated MW of 35.6kDa according to NP_001632.2; NP_542379.1; NP_542380.1. Recommended concentration: 0.5-2µg/ml.
Species ReactivityTested: Human
Expected from sequence similarity: Human
Background ReferenceDemple B, Herman T, Chen DS.
Cloning and expression of APE, the cDNA encoding the major human apurinic endonuclease: definition of a family of DNA repair enzymes.
Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11450-4.
PMID: 1722334
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB05345 staining (0.5µg/ml) of A431 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
