EB05315-T Goat Anti-RACGAP1 / MgcRacGAP Antibody - Trial Size
Price: $100.00 / €100,00 / £75.00
Size: 20µg specific antibody in 40µl
EU Availability: In stock
US Availability: In stock
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EB05315 Goat Anti-RACGAP1 / MgcRacGAP Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
Click to add to EURO Shopping Cart (EU customers only)
Click to add to US Dollar Shopping Cart (US customers only)
Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: RACGAP1; Rac GTPase activating protein 1; ID-GAP; MGCRACGAP; MgcRacGAP; GTPase activating protein; HsCYK-4
Official Symbol: RACGAP1
Accession Number(s): NP_037409.1
Human GeneID(s): 29127
Gene Ontology Terms: intracellular signaling cascade
Immunogen Peptide with sequence C-GRQGNFFASPMLK, from the C Terminus of the protein sequence according to NP_037409.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedWestern blot: Approx 60kDa band seen in Human Testis lysate. Recommended for use at 0.2-1µg/ml. Please note that we observe a 60kDa band as suggested in the literature (for example, see Toure et al., [J Biol Chem 1998 Mar 13;273(11):6019-23]), which is different to the predicted MW of approx. 70kDa.
Immunohistochemistry: In paraffin embedded Human Testis, shows nulear staining of many cells. Recommended concentration, 1µg/ml.Species ReactivityTested: Human
Expected from sequence similarity: Human
Product Specific ReferenceThe goat polyclonal antibody used in this paper was manufactured by us:
Nishimura Y et al. Centralspindlin regulates ECT2 and RhoA accumulation at the equatorial cortex during cytokinesis. J Cell Sci. 2006 Jan 1;119(Pt 1):104-14. Epub 2005 Dec 13.
PMID: 16352658
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB05315 staining (0.2µg/ml) of Human Testis lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB05315 (1µg/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. |
