EB05310-T Goat Anti-Calretinin Antibody - Trial Size Price: $100.00 / €100,00 / £75.00 Size: 20µg specific antibody in 40µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info EB05310 Goat Anti-Calretinin Antibody Price: $285.00 / €292,50 / £195.00 Size: 100µg specific antibody in 200µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: Calretinin; ANXA2; annexin A2; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; P36; PAP-IV; annexin II; lipocortin II; Annexin II (lipocortin I); annexin II (lipocortin II); calpactin I, heavy polypeptide (p36); annexin II (lipocortin II calpactin I, heavy polypeptide); calpactin I heavy polypeptide; chromobindin 8; lipocortin II; placental anticoagulant protein IV Official Symbol: ANXA2 Accession Number(s): NP_001002858.1; NP_004030.1; NP_001002857.1 Human GeneID(s): 302 Non-Human GeneID(s): 12306 (mouse); 56611 (rat); Gene Ontology Terms: phospholipase inhibitor activity; calcium-dependent phospholipid binding; calcium ion binding; skeletal development; soluble fraction; plasma membrane; anticoagulant activity Important Comments: This antibody is expected to recognise both reported isoforms. NP_004030.1 and NP_001002857.1 are varients of isoform 2. Immunogen Peptide with sequence STVHEILCKLSLEGD, from the N Terminus of the protein sequence according to NP_001002858.1; NP_004030.1; NP_001002857.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Approx 35kDa band observed in lysates of cell lines A431 and A549 (calculated MW of 38.6kDa according to NP_004030.1 and NP_001002857.1). Recommended concentration: 1-3µg/ml.
Species ReactivityTested: Human Expected from sequence similarity: Human, Mouse, Rat, Cow, Pig Background ReferenceTakahashi S, Reddy SV, Chirgwin JM, Devlin R, Haipek C, Anderson J, Roodman GD. Cloning and identification of annexin II as an autocrine/paracrine factor that increases osteoclast formation and bone resorption. J Biol Chem 1994 Nov 18;269(46):28696-701 PMID: 7961821
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For Research purposes only. Not for diagnostic or in vivo use. © Copyright Everest Biotech Ltd 2009 | Elite Grade Product 


EB05310 staining (1µg/ml) of A431 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |