EB05292-T Goat Anti-SIAH1 Antibody - Trial Size
Price: $100.00 / €100,00 / £75.00
Size: 20µg specific antibody in 40µl
EU Availability: In stock
US Availability: In stock
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EB05292 Goat Anti-SIAH1 Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
Click to add to EURO Shopping Cart (EU customers only)
Click to add to US Dollar Shopping Cart (US customers only)
Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: SIAH1; seven in absentia homolog 1 (Drosophila); Siah-1; hSIAH1; HUMSIAH; Siah-1a
Official Symbol: SIAH1
Accession Number(s): NP_003022.3; NP_001006611.1
Human GeneID(s): 6477
Non-Human GeneID(s): 20437 (mouse);
Gene Ontology Terms: axon guidance; proteolysis and peptidolysis; ubiquitin-dependent protein catabolism; embryogenesis and morphogenesis; development; neurogenesis; apoptosis; cytoplasm; nucleus
Important Comments: This product is expected to recognize both reported isoforms (according to NP_003022.3; NP_001006611.1) Immunogen Peptide with sequence SRQTATALPTGTSKC, from the N Terminus of the protein sequence according to NP_003022.3; NP_001006611.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 28+30kDa bands observed in Human Liver lysates (calculated MW of 31.1kDa and 34.6kDa according to NP_003022.3 and NP_001006611.1). Recommended concentration: 0.1-0.3µg/ml.
Species ReactivityTested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog
Product Specific ReferenceThe goat polyclonal antibody used in this paper was manufactured by us:
Iwai A et al. Siah-1L, a novel transcript variant belonging to the human Siah family of
proteins, regulates beta-catenin activity in a p53-dependent manner. Oncogene. 2004 Sep 30;23(45):7593-600.
PMID: 15326481
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB05292 staining (0.1µg/ml) of Human Liver lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
