EB05291-T Goat Anti-ASC / TMS1 Antibody - Trial Size
Price: $100.00 / €100,00 / £75.00
Size: 20µg specific antibody in 40µl
EU Availability: In stock
US Availability: In stock
Click to add to EURO Shopping Cart (EU customers only)
Click to add to US Dollar Shopping Cart (US customers only)
Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
EB05291 Goat Anti-ASC / TMS1 Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
Click to add to EURO Shopping Cart (EU customers only)
Click to add to US Dollar Shopping Cart (US customers only)
Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: ASC; TMS1; PYCARD; PYD and CARD domain containing; CARD5; MGC10332; TMS; apoptosis-associated speck-like protein containing a CARD; caspase recruitment domain protein 5; target of methylation-induced silencing-1
Official Symbol: PYCARD
Accession Number(s): NP_037390.2; NP_660183.1; NP_660184.1
Human GeneID(s): 29108
Gene Ontology Terms: caspase activation; caspase activator activity; cytoplasm; induction of apoptosis; negative regulation of cell cycle; protein binding; regulation of apoptosis; signal transduction;
Important Comments: This antibody is expected to recognise all three reported Human isoforms according to NP_037390.2; NP_660183.1 and NP_660184.1. Immunogen Peptide with sequence C-RESQSYLVEDLERS, from the C Terminus of the protein sequence according to NP_037390.2; NP_660183.1; NP_660184.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx. 22kDa band observed in Hela and Human Kidney lysates (predicted MW of 22kDa according to NP_037390.2). Recommended for use at 1-3µg/ml.
Species ReactivityTested: Human
Expected from sequence similarity: Human
Background ReferenceOhtsuka T, Ryu H, Minamishima YA, Macip S, Sagara J, Nakayama KI, Aaronson SA, Lee SW.
ASC is a Bax adaptor and regulates the p53-Bax mitochondrial apoptosis pathway.
Nat Cell Biol. 2004 Feb;6(2):121-8
PMID: 14730312
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB05291 (1µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing ASC (RC215592) and probed with EB05291 (mock transfection in first lane). |
