EB05247-T Goat Anti-ATF4 Antibody - Trial Size Price: $100.00 / €100,00 / £75.00 Size: 20µg specific antibody in 40µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info EB05247 Goat Anti-ATF4 Antibody Price: $285.00 / €292,50 / £195.00 Size: 100µg specific antibody in 200µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: ATF4; activating transcription factor 4 (tax-responsive enhancer element B67); CREB2; TXREB; CREB-2; TAXREB67 Official Symbol: ATF4 Accession Number(s): NP_001666 Human GeneID(s): 468 Non-Human GeneID(s): 11911 (mouse); 79255 (rat); Gene Ontology Terms: RNA polymerase II transcription factor activity; DNA binding; regulation of transcription, DNA-dependent; nucleus Immunogen Peptide with sequence C-EEVRKARGKKRVP, from the C Terminus of the protein sequence according to NP_001666. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedWestern blot: Approx 40kDa band seen in Raji cell lysate. Recommended for use at 1-3 µg/ml.
Species ReactivityTested: Human Expected from sequence similarity: Human, Mouse, Rat Background ReferenceTsujimoto A, Nyunoya H, Morita T, Sato T, Shimotohno K. Isolation of cDNAs for DNA-binding proteins which specifically bind to a tax-responsive enhancer element in the long terminal repeat of human T-cell leukemia virus type I. J Virol 1991 Mar;65(3):1420-6 PMID: 1847461
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EB05247 staining (1µg/ml) of Raji lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |