EB05230 Goat Anti-PPP2CA / PPP2CB Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: PPP2CA; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; PP2CA; PPP2CA; protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform; PP2CB
Official Symbol: PPP2CA / PPP2CB
Accession Number(s): NP_002706.1; NP_004147.1
Human GeneID(s): 5515; 5516
Non-Human GeneID(s): 19052 (mouse); 24672 (rat);
Gene Ontology Terms: protein phosphatase type 2A, intrinsic catalyst activity; protein phosphatase type 2C activity; regulation of cell cycle; protein amino acid dephosphorylation; protein phosphatase type 2A complex; calcium-dependent protein serine/threonine phosphatase activity; magnesium-dependent protein serine/threonine phosphatase activity; CTD phosphatase activity; hydrolase activity; myosin phosphatase activity; manganese ion binding; protein phosphatase type 2B activity
Immunogen Peptide with sequence C-EPHVTRRTPDYFL, from the C Terminus of the protein sequence according to NP_002706.1; NP_004147.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 35kDa band observed in Human Brain (Cerebellum), Mouse Brain and Rat Brain lysates (calculated MW of 35.6kDa according to NP_002706.1and NP_004147.1). Recommended concentration: 0.05-0.2µg/ml.
Species ReactivityTested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Rabbit, Pig
Background ReferenceHemmings BA, Wernet W, Mayer R, Maurer F, Hofsteenge J, Stone SR.
The nucleotide sequence of the cDNA encoding the human lung protein phosphatase 2A beta catalytic subunit.
Nucleic Acids Res 1988 Dec 9;16(23):11366
PMID: 2849765
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB05230 (0.05µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
