EB05227 Goat Anti-NFIL3 Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: NFIL3; nuclear factor, interleukin 3 regulated; E4BP4; IL3BP1; NFIL3A; NF-IL3A; interleukin-3 promoter transcriptional activator
Official Symbol: NFIL3
Accession Number(s): NP_005375.2
Human GeneID(s): 4783
Non-Human GeneID(s): 18030 (mouse); 114519 (rat);
Gene Ontology Terms: transcription co-repressor activity; transcription factor activity; regulation of transcription, DNA-dependent; immune response; transcription from Pol II promoter; nucleus
Immunogen Peptide with sequence C-RLIATQPISASDSG, from the C Terminus of the protein sequence according to NP_005375.2. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx. 55-60kDa band observed in lysates of Jurkat and HepG2 (calculated MW of 51.5kDa according to NP_005375.2). The observed molecular weight corresponds to earlier findings in literature (Ikushima et al., Proc Natl Acad Sci U S A. 1997 Mar 18;94(6):2609-14.; PMID: 9122243). Recommended concentration: 0.5-1.5µg/ml.
Species ReactivityTested: Human
Expected from sequence similarity: Human, Mouse, Rat
Background ReferenceZhang W, Zhang J, Kornuc M, Kwan K, Frank R, Nimer SD.
Molecular cloning and characterization of NF-IL3A, a transcriptional activator of the human interleukin-3 promoter.
Mol Cell Biol 1995 Nov;15(11):6055-63
PMID: 7565758
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB05227 staining (0.5µg/ml) of HepG2 lysate (RIPA buffer, 20µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
