EB05198-T Goat Anti-ATF2 Antibody - Trial Size Price: $100.00 / €100,00 / £75.00 Size: 20µg specific antibody in 40µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info EB05198 Goat Anti-ATF2 Antibody Price: $285.00 / €292,50 / £195.00 Size: 100µg specific antibody in 200µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: ATF2; activating transcription factor 2; HB16; CREB2; TREB7; CRE-BP1; cAMP responsive element binding protein 2; MGC111558 Official Symbol: ATF2 Accession Number(s): NP_001871.2 Human GeneID(s): 1386 Non-Human GeneID(s): 81647 (rat); Gene Ontology Terms: RNA polymerase II transcription factor activity; transcription co-activator activity Immunogen Peptide with sequence C-IVMAPSSQSQPSGS, from the C Terminus of the protein sequence according to NP_001871.2. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: experiments gave bands at approx 55kDa and 30kDa in lysates of Human Placenta and of cell line HeLa after 0.5µg/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (Bailey et al , J Clin Endocrinol Metab. 2002 Apr;87(4):1717-28.; PMID: 11932306). This protein has a calculated MW of 54.5kDa according to NP_001871.2. Recommended concentration: 0.5-1.5µg/ml.
Species ReactivityTested: Human Expected from sequence similarity: Human, Rat Background ReferenceMonzen K, Hiroi Y, Kudoh S, Akazawa H, Oka T, Takimoto E, Hayashi D, Hosoda T, Kawabata M, Miyazono K, Ishii S, Yazaki Y, Nagai R, Komuro I. Smads, TAK1, and their common target ATF-2 play a critical role in cardiomyocyte differentiation. J Cell Biol 2001 May 14;153(4):687-98 PMID: 11352931
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EB05198 staining (0.5µg/ml) of Human Placenta lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence. |