EB05197 Goat Anti-DUSP1 Antibody Price: $285.00 / €292,50 / £195.00 Size: 100µg specific antibody in 200µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: DUSP1; dual specificity phosphatase 1; HVH1; CL100; MKP-1; PTPN10; serine/threonine specific protein phosphatase Official Symbol: DUSP1 Accession Number(s): NP_004408.1 Human GeneID(s): 1843 Non-Human GeneID(s): 19252 (mouse); Gene Ontology Terms: protein phosphatase type 2C activity; protein phosphatase type 2A activity; non-membrane spanning protein tyrosine phosphatase activity; cell cycle; response to oxidative stress; protein amino acid dephosphorylation; calcium-dependent protein serine/threonine phosphatase activity; magnesium-dependent protein serine/threonine phosphatase activity; CTD phosphatase activity; hydrolase activity; MAP kinase phosphatase activity; myosin phosphatase activity; protein phosphatase type 2B activity Immunogen Peptide with sequence SYLQSPITTSPSC, from the C Terminus of the protein sequence according to NP_004408.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx. 40kDa band observed in Human lysates of HeLa (calculated MW of 39.3kDa according to NP_004408.1). Recommended concentration: 0.3-1µg/ml.
Species ReactivityTested: Human Expected from sequence similarity: Human, Mouse, Rat Background ReferenceAlessi DR, Smythe C, Keyse SM. The human CL100 gene encodes a Tyr/Thr-protein phosphatase which potently and specifically inactivates MAP kinase and suppresses its activation by oncogenic ras in Xenopus oocyte extracts. Oncogene 1993 Jul;8(7):2015-20 PMID: 8390041
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For Research purposes only. Not for diagnostic or in vivo use. © Copyright Everest Biotech Ltd 2009 | Elite Grade Product 

EB05197 (0.3µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |