EB05195 Goat Anti-MTM1 Antibody Price: $285.00 / €292,50 / £195.00 Size: 100µg specific antibody in 200µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: MTM1; myotubular myopathy 1; CNM; MTMX; XLMTM; myotubularin Official Symbol: MTM1 Accession Number(s): NP_000243.1 Human GeneID(s): 4534 Gene Ontology Terms: protein serine/threonine phosphatase activity; prenylated protein tyrosine phosphatase activity; cell growth and/or maintenance; muscle development; protein amino acid dephosphorylation; cellular_component unknown; hydrolase activity Immunogen Peptide with sequence C-SSPSQMMPHVQTHF, from the C Terminus of the protein sequence according to NP_000243.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 70kDa band observed in lysates of Human Skeletal Muscle and of cell line HepG2 (calculated MW of 69.9kDa according to NP_000243.1). Recommended concentration: 0.3-1µg/ml.
Species ReactivityTested: Human Expected from sequence similarity: Human Background ReferenceLaporte J, Hu LJ, Kretz C, Mandel JL, Kioschis P, Coy JF, Klauck SM, Poustka A, Dahl N. A gene mutated in X-linked myotubular myopathy defines a new putative tyrosine phosphatase family conserved in yeast. Nat Genet. 1996 Jun;13(2):175-82. PMID: 8640223
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For Research purposes only. Not for diagnostic or in vivo use. © Copyright Everest Biotech Ltd 2009 | Elite Grade Product 

EB05195 (0.3µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |