EB05054-T Goat Anti-NCF1 / p47phox Antibody - Trial Size Price: $100.00 / €100,00 / £75.00 Size: 20µg specific antibody in 40µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info EB05054 Goat Anti-NCF1 / p47phox Antibody Price: $285.00 / €292,50 / £195.00 Size: 100µg specific antibody in 200µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: NCF1; p47phox; FLJ79451; NCF1A; NOXO2; SH3PXD1A; neutrophil cytosolic factor 1; Neutrophil cytosolic factor-1 (47kD); neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1); NADPH oxidase organizer 2 Official Symbol: NCF1 Accession Number(s): NP_000256.3 Human GeneID(s): 653361 Non-Human GeneID(s): 17969 (mouse); 114553 (rat); Gene Ontology Terms: GTPase activity; protein binding; electron transporter activity; electron transport; superoxide metabolism; cellular defense response; intracellular signaling cascade; cytosol; soluble fraction Immunogen Peptide with sequence C-SESTKRKLASAV, from the C Terminus of the protein sequence according to NP_000256.3. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedWestern blot: Approx 47kDa band seen in U937 cell lysates. Recommended for use at 0.2-2µg/ml.
Species ReactivityTested: Human Expected from sequence similarity: Human, Mouse, Rat, Rabbit, Cow Product Specific ReferenceThe goat polyclonal antibody used in this paper was manufactured by us: Underhill DM et al. Dectin-1 activates Syk tyrosine kinase in a dynamic subset of macrophages for reactive oxygen production. Blood. 2005 Oct 1;106(7):2543-50. Epub 2005 Jun 14. PMID: 15956283
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For Research purposes only. Not for diagnostic or in vivo use. © Copyright Everest Biotech Ltd 2009 | Elite Grade Product 


EB05054 staining (0.2µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |